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Home: Perl Programming Help: Beginner:
text search in file

 


cmccabe1
Novice

Jul 9, 2013, 9:48 AM

Post #1 of 13 (1886 views)
text search in file Can't Post
I have a (test.vcf) file in which the INFO column is always 5th and looks like:



RS=199476396;RSPOS=985955;dbSNPBuildID=136;SSR=0;SAO=1;VP=0x050260000a01000002110100;GENEINFO=AGRN:375790;WGT=1;VC=SNV;PM;S3D;NSM;REF;OTHERKG;LSD;OM;CLNALLE=1;CLNHGVS=NC_000001.10:g.985955G>C;CLNSRC=OMIM Allelic Variant;CLNORIGIN=1;CLNSRCID=103320.0001;CLNSIG=5;CLNDSDB=GeneReviews:NCBI:OMIM:Orphanet;CLNDSDBID=NBK1168:C1850792:254300:590;CLNDBN=Myasthenia\x2c limb-girdle\x2c familial;CLNACC=RCV000019902.1

RS=207460006;RSPOS=1199489;dbSNPBuildID=136;SSR=0;SAO=0;VP=0x050060080001000002110100;GENEINFO=UBE2J2:118424;WGT=1;VC=SNV;PM;INT;OTHERKG;LSD;OM;CLNALLE=1;CLNHGVS=NC_000001.10:g.1199489G>A;CLNSRC=.;CLNORIGIN=2;CLNSRCID=.;CLNSIG=1;CLNDSDB=.;CLNDSDBID=.;CLNDBN=.;CLNACC=.

RS=144003672;RSPOS=1245104;dbSNPBuildID=134;SSR=0;SAO=2;VP=0x050268020a01000002100120;GENEINFO=ACAP3:116983|PUSL1:126789;WGT=1;VC=SNV;PM;PMC;S3D;NSM;REF;R5;OTHERKG;LSD;CLNALLE=1;CLNHGVS=NC_000001.10:g.1245104C>A;CLNSRC=.;CLNORIGIN=2;CLNSRCID=.;CLNSIG=1;CLNDSDB=.;CLNDSDBID=.;CLNDBN=.;CLNACC=.

RS=145324009;RSPOS=1469331;dbSNPBuildID=134;SSR=0;SAO=2;VP=0x050268000a01000002100120;GENEINFO=ATAD3A:55210;WGT=1;VC=SNV;PM;PMC;S3D;NSM;REF;OTHERKG;LSD;CLNALLE=1;CLNHGVS=NC_000001.10:g.1469331G>A;CLNSRC=.;CLNORIGIN=2;CLNSRCID=.;CLNSIG=1;CLNDSDB=.;CLNDSDBID=.;CLNDBN=.;CLNACC=.



Out of each line all that is needed is RS= up to the ;, GENEINFO= up to the ;, CLNHGVS= up to the ;, CLNSIG= up to the ;, CLNDBN= up to the ;. Is there a command that can do this? Thank you.


FishMonger
Veteran / Moderator

Jul 9, 2013, 10:05 AM

Post #2 of 13 (1883 views)
Re: [cmccabe1] text search in file [In reply to] Can't Post
Yes, you should use the split function along with an array slice to extract those 5 fields. Then, if needed, use the split function on those fields to separate the 'key'='value' pairs.

Here's an example using your data.
Code
#!/usr/bin/perl 
 
use strict; 
use warnings; 
use Data::Dumper; 
 
$/ = ''; 
 
while (my $line = <DATA>) { 
    my @fields = (split(/;/, $line))[0,6,17,21,24]; 
    print Dumper \@fields; 
} 
 
__DATA__ 
RS=199476396;RSPOS=985955;dbSNPBuildID=136;SSR=0;SAO=1;VP=0x050260000a01000002110100;GENEINFO=AGRN:375790;WGT=1;VC=SNV;PM;S3D;NSM;REF;OTHERKG;LSD;OM;CLNALLE=1;CLNHGVS=NC_000001.10:g.985955G>C;CLNSRC=OMIM Allelic Variant;CLNORIGIN=1;CLNSRCID=103320.0001;CLNSIG=5;CLNDSDB=GeneReviews:NCBI:OMIM:Orphanet;CLNDSDBID=NBK1168:C1850792:254300:590;CLNDBN=Myasthenia\x2c limb-girdle\x2c familial;CLNACC=RCV000019902.1 
 
RS=207460006;RSPOS=1199489;dbSNPBuildID=136;SSR=0;SAO=0;VP=0x050060080001000002110100;GENEINFO=UBE2J2:118424;WGT=1;VC=SNV;PM;INT;OTHERKG;LSD;OM;CLNALLE=1;CLNHGVS=NC_000001.10:g.1199489G>A;CLNSRC=.;CLNORIGIN=2;CLNSRCID=.;CLNSIG=1;CLNDSDB=.;CLNDSDBID=.;CLNDBN=.;CLNACC=. 
 
RS=144003672;RSPOS=1245104;dbSNPBuildID=134;SSR=0;SAO=2;VP=0x050268020a01000002100120;GENEINFO=ACAP3:116983|PUSL1:126789;WGT=1;VC=SNV;PM;PMC;S3D;NSM;REF;R5;OTHERKG;LSD;CLNALLE=1;CLNHGVS=NC_000001.10:g.1245104C>A;CLNSRC=.;CLNORIGIN=2;CLNSRCID=.;CLNSIG=1;CLNDSDB=.;CLNDSDBID=.;CLNDBN=.;CLNACC=. 
 
RS=145324009;RSPOS=1469331;dbSNPBuildID=134;SSR=0;SAO=2;VP=0x050268000a01000002100120;GENEINFO=ATAD3A:55210;WGT=1;VC=SNV;PM;PMC;S3D;NSM;REF;OTHERKG;LSD;CLNALLE=1;CLNHGVS=NC_000001.10:g.1469331G>A;CLNSRC=.;CLNORIGIN=2;CLNSRCID=.;CLNSIG=1;CLNDSDB=.;CLNDSDBID=.;CLNDBN=.;CLNACC=.
Outputs:
Code
$VAR1 = [ 
          'RS=199476396', 
          'GENEINFO=AGRN:375790', 
          'CLNHGVS=NC_000001.10:g.985955G>C', 
          'CLNSIG=5', 
          'CLNDBN=Myasthenia\\x2c limb-girdle\\x2c familial' 
        ]; 
$VAR1 = [ 
          'RS=207460006', 
          'GENEINFO=UBE2J2:118424', 
          'CLNORIGIN=2', 
          'CLNDSDBID=.', 
          undef 
        ]; 
$VAR1 = [ 
          'RS=144003672', 
          'GENEINFO=ACAP3:116983|PUSL1:126789', 
          'CLNALLE=1', 
          'CLNSRCID=.', 
          'CLNDSDBID=.' 
        ]; 
$VAR1 = [ 
          'RS=145324009', 
          'GENEINFO=ATAD3A:55210', 
          'CLNHGVS=NC_000001.10:g.1469331G>A', 
          'CLNSIG=1', 
          'CLNDBN=.' 
        ];


FishMonger
Veteran / Moderator

Jul 9, 2013, 10:09 AM

Post #3 of 13 (1881 views)
Re: [cmccabe1] text search in file [In reply to] Can't Post
I should point out that format of your data needs to be consistent for this approach to work. If it's not, then you'll need to use one or more regex's to extract the data. As you can see from the output of my example, the format of the 2nd and 3rd records are not consistent with the other 2.


(This post was edited by FishMonger on Jul 9, 2013, 10:10 AM)


cmccabe1
Novice

Jul 9, 2013, 10:17 AM

Post #4 of 13 (1876 views)
Re: [FishMonger] text search in file [In reply to] Can't Post
I am new to PERL but if I create a .pl file with the code in it. How can I run that script, what command do I use or is there another way? Thank you.


FishMonger
Veteran / Moderator

Jul 9, 2013, 10:27 AM

Post #5 of 13 (1872 views)
Re: [cmccabe1] text search in file [In reply to] Can't Post
Are you on windows or a *nix system?

I'll assume you're on Windows. Think of this perl script as if it were a batch script and execute the same way.

For example, assuming the script is named example.pl
----------------------------------
c:\test>example.pl
or
c:\test>perl example.pl


cmccabe1
Novice

Jul 9, 2013, 10:41 AM

Post #6 of 13 (1866 views)
Re: [FishMonger] text search in file [In reply to] Can't Post
I am using Cygwin on a windows OS.

I created a vcfsplit.pl file with the code in it.

Thank you.


Laurent_R
Veteran / Moderator

Jul 9, 2013, 10:52 AM

Post #7 of 13 (1862 views)
Re: [cmccabe1] text search in file [In reply to] Can't Post
You can either change the file permissions to 755 (with the "chmod 777 example.pl" bash command) and then run as:

Code
./example.pl
provided you have this "shebang" line at the top of your program:

Code
#!/usr/bin/perl
Or, even simpler:

Code
perl example.pl


cmccabe1
Novice

Jul 9, 2013, 11:57 AM

Post #8 of 13 (1850 views)
Re: [Laurent_R] text search in file [In reply to] Can't Post
I am getting this error:

Name "main::DATA" used only once: possible typo at ./splitvcf.pl line 9.
readline() on unopened filehandle DATA at ./splitvcf.pl line 9. Thank you.


Laurent_R
Veteran / Moderator

Jul 9, 2013, 11:16 PM

Post #9 of 13 (1838 views)
Re: [cmccabe1] text search in file [In reply to] Can't Post
Did you copy in your program the __DATA__ section used by Fishmonger at the end of his example?


cmccabe1
Novice

Jul 10, 2013, 6:22 AM

Post #10 of 13 (1828 views)
Re: [Laurent_R] text search in file [In reply to] Can't Post
I placed the __DATA__ section under the code:

Here is the error I get:
perl splitvcf.pl >output.txt
Can't open perl script "splitvcf.pl": No such file or directory

the splitvcf.pl is in the home directory of cygwin.

Thank you.
In Reply To


FishMonger
Veteran / Moderator

Jul 10, 2013, 7:13 AM

Post #11 of 13 (1822 views)
Re: [cmccabe1] text search in file [In reply to] Can't Post
I've never been much of a fan of cygwin.

Are you executing it from within the cygwin shell?

Are you in that same directory as the script when you execute it, or are you in some other directory?

Try specifying the full path.


cmccabe1
Novice

Jul 10, 2013, 7:27 AM

Post #12 of 13 (1815 views)
Re: [FishMonger] text search in file [In reply to] Can't Post
That was it the command runs, but I only get output from the first record. Since the format may different in each field as you suggested in your third post. Is it possible to use RS= up to the ;, GENEINFO= up to the ;, CLNHGVS= up to the ;, CLNSIG= up to the ;, CLNDBN= up to the ; and if that text is not there it is undefined or blank? Thank you for all your help.


FishMonger
Veteran / Moderator

Jul 10, 2013, 8:17 AM

Post #13 of 13 (1811 views)
Re: [cmccabe1] text search in file [In reply to] Can't Post
If you have inconsistent data format, then you'll need to use a regex. Please read over the info in the following perldocs.

perlrequick - Perl regular expressions quick start
http://perldoc.perl.org/5.16.2/perlrequick.html

perlretut - Perl regular expressions tutorial
http://perldoc.perl.org/5.16.2/perlretut.html

Once you read over that info, try to write a regex that extracts the info you need. Your first tries may not work but you can then post your efforts and we will help you to fix them.

 
 


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